Description: The fish line CHSE/F was derived from Common bluegill (Lepomis macrochirus) embryo. It is susceptible to various fish viruses including infectious hematopoietic necrosis virus (IHNV); viral haemorrhagic septicaemia virus (VHSV); spring viraemia of carp virus (SVCV); and infectious pancreatic necrosis virus (IPNV). ECACC has been advised by the CCLV that this cell line is derived from Lepomis macrochirus (Common bluegill) not Oncorhynchus tshawytscha (Chinook salmon) as originally reported. This has been confirmed by DNA sequencing at ECACC. The CCLV have changed the cell line name to CHSE/F to indicate this. The line is used in viral diagnostics and gives better results than BF-2 (ECACC Catalogue number 00021712)”, a commonly used cell line of Lepomis macrochirus. The sequence of both lines (BF-2 and CHSE/F possess the same nucleotide exchange but are different in culture behaviour and in virus propagation.

Also Known As:

Species: Fish

Tissue: Embryo

Growth Properties: Adherent

Morphology: Fibroblast-like

Growth Medium: MEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 120mg/l Sodium Pyruvate (NaPy) + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:4 to 1:6 using 0.25% trypsin or trypsin/EDTA; grow cells in dark (e.g. wrapped in foil) at 20-26°C with 26°C optimum temperature; monolayer is stable for at least 10 days with maintenance medium containing 1-2% FBS. Freeze in 95% FBS with 5% DMSO. Fish cell lines detach easily during transit if the culture is too young. After resuscitation split cells 1:4 and culture for at least one week with 1-2 media changes before shipping.

Release Conditions: No

Depositor:

Originator: N

References: Ann NY Acad Sci 1965; 126:566, In Vitro 1984; 20:671

Hyperlink to ECACC Cell Line Data Sheet: 00021714