CLC
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Description
Description: The cell line CLC (Carp Leukocyte Culture) was established from peripheral blood mononuclear cells of a normal 2 year old female common carp, Cyprinus carpio. They possess phagocytic activity and are reported to be stimulated by TNF, gamma-interferon and LPS therefore resembling characteristic features of monocytes / macrophages. CLC replicate fish viruses e.g. IPNV, PFR, SVCV and VHSV.
Also Known As:
Species: Fish
Tissue: blood
Growth Properties: Adherent
Morphology: Epithelial-like
Growth Medium: EMEM (EBSS) or TC-199 + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Spilt confluent cultures 1:3 using 0.25% trypsin or trypsin/EDTA; 25-28°C. Fish cell lines detach easily during transit if the culture is too young. After resuscitation split cells 1:3 and culture for at least one week with 1-2 media changes before shipping.
Release Conditions: No
Depositor: Prof W Ahne, Institute of Zoology, Fishery Biology and Fish Diseases, University of Munich
Originator: Yes
References: Dev Comp Immunol 1990;14:255
Hyperlink to ECACC Cell Line Data Sheet: 95070628
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