Description: Derived from a ten year old male golden retriever with malignant histiocytosis. The cells have a macrophage-like morphology and are able to phagocytose latex particles. They are positive for Fc-gamma receptors and are negative for Fc-mu and C3b receptors. DH82 cells do not produce IL-1.

Also Known As:

Species: Dog

Tissue: macrophage

Growth Properties: Suspension

Morphology: Macrophage

Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS) (Heat Inactivated).

Subculturing Procedure: Cells are semi-adherent, i.e. some cells grows in suspension, whilst most attach to the surface and may flatten. Attached cells should be removed with 0.02% EDTA. Split sub-confluent cultures (70-80%); i.e. seeding at 2-4×10,000 cells/cm²; 5% CO2; 37°C.

Release Conditions: No

Depositor:

Originator: No

References: Wellman ML, Krakowka S, Jacobs RM, Kociba GJ 1988 A macrophage-monocyte cell line from a dog with malignant histiocytosis. In Vitro Cell Dev Biol.24(3):223-9. PMID: 3350786.

Hyperlink to ECACC Cell Line Data Sheet: 94062922