GS-109-V-63
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Description
Description: Derived from a 13 year old Caucasian male with Gardner’s syndrome, an autosomal dominant condition which predisposes to carcinoma and multiple polyps of the colon. The modal chromosome number was 46, occurring in 60% of cells.
Also Known As:
Species: Human
Tissue: skin
Growth Properties: Adherent
Morphology: Fibroblast
Growth Medium: EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 20% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split semi-confluent cultures 1:2 i.e. seeding at 4×10, 000 cells/cm² using 0.25% trypsin; 5% CO2; 37°C. Monolayer should form in a 25cm² flask in 3-4 days.
Release Conditions: No
Depositor:
Originator: No
References: None specified by depositor
Hyperlink to ECACC Cell Line Data Sheet: 90110503
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