HEPM
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Description
Description: Established from embryonic palatal shelves. The cells are very sensitive to the stimulating effect of EGF with respect to proliferation, incorporation of thymidine and ornithine decarboxylase.
Also Known As:
Species: Human
Tissue: Palate, embryo
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10, 000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: Science 1981;213:563
Hyperlink to ECACC Cell Line Data Sheet: 90120505
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