HL60 15-12
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Description
Description: HL60 15-12 is a variant of HL60 which is capable of differentiating towards either neutrophils or monocytes with treatment of appropriate agents.
Also Known As:
Species: Human
Tissue: blood
Growth Properties: Suspension
Morphology:
Growth Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 1.25% Dimethyl Sulphoxide (DMSO).
Subculturing Procedure: Maintain cultures between 2×100,000 to 1,0,000 cells/ml. NB: The cells will differentiate if the culture medium is allowed to become acidic. 5% CO2; 37°C. Freeze in 10% glycerol and 90% FBS.
Release Conditions: No
Depositor: Dr G Brown/Dr C M Bunce, Immunology Department The Medical School, University of Birmingham
Originator: Yes
References: Br J Cancer 1986;57:559
Hyperlink to ECACC Cell Line Data Sheet: 88120805
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