HR5-CL11
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Description
Description: HR5-CL11 cells were obtained after transfection of HeLa cells with plasmids coding for a transcriptional transactivator and transferring G418 and hygromycin resistance. This tetracycline-controlled transactivator (a fusion between the tet repressor and the activating domain of the herpes simplex virus VP16 transcription activation domain) can stimulate the expression of a reporter gene up to 100,000 fold. Subsequently these cells were stably transfected with a luciferase reporter unit. Luciferase expression in the HR5-CL11 cells is highly stimulated by doxycycline.
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Species: Human
Tissue: cervix
Growth Properties: Adherent
Morphology: Epithelial-like
Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:5 to 1:20 i.e. seeding at 2×1,000 to 2×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor: Prof H Bujard, ZMBH, Heidelberg
Originator: Yes
References: Science 1995;268:1766
Hyperlink to ECACC Cell Line Data Sheet: 95091411
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