Hs1.Tes
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Description
Description: Normal human testis. Cell line may have a finite life span, therefore passage number should be kept as low as possible. Population doublings have not been determined.
Also Known As:
Species: Human
Tissue: Testis
Growth Properties: Adherent
Morphology: Fibroblast
Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5×10, 000 cells/cm² using 0.25% trypsin; 5% CO2; 37°C. This cell line may have a finite life span therefore the population doubling time should be calculated at every passage to ensure cells are not reaching senescence.
Release Conditions: No
Depositor:
Originator: No
References: None specified by depositor
Hyperlink to ECACC Cell Line Data Sheet: 97123004
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