IEC 6
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Description
Description: Normal rat small intestine epithelial cells which synthesise fibronectin and collagen. Growth is inhibited by cortisol. Cells possess cell surface antigens specific for intestinal epithelial cells in vivo. Capable of at least 10 population doublings.
Also Known As:
Species: Rat
Tissue: intestine
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: DMEM + 2mM Glutamine + 0.1 IU/ml Insulin + 5% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor: Obtained from ATCC
Originator: No
References: J Cell Biol 1979;80:248
Hyperlink to ECACC Cell Line Data Sheet: 88071401
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