JTC-27
Rat hepatoma
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Description
Description: Derived from a rat ascites hepatoma.
Also Known As:
Species: Rat
Tissue: Ascites
Growth Properties: Adherent
Morphology: Epithelial-like
Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (10% for initial cell attachment after thawing).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:8 i.e. seeding at 1×10000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: J Exp Med 1972;42:249
Hyperlink to ECACC Cell Line Data Sheet: 94042255
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