L6.C11
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Description
Description: A subclone of L6, a rat thigh muscle cell line. On reaching confluence the cells will fuse to form myotubes and twitching movement will be seen. Once cultures have fused they cannot be recloned.
Also Known As:
Species: Rat
Tissue: muscle
Growth Properties: Adherent
Morphology: Fibroblast
Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split semi-confluent cultures to a seeding density of 1.5-2×1,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C. Cells fuse on reaching confluence, producing myotubes. To prevent fusion maintain the cultures sub-confluent and reclone every 6-8 weeks.
Release Conditions: No
Depositor:
Originator: No
References: Proc Natl Acad Sci, USA 1968;61:477; Dev Biol 1970;23:1
Hyperlink to ECACC Cell Line Data Sheet: 92102119
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