Description: Derived from mononuclear cells from peripheral blood of a 51 year old normal male. Cells were subsequently transformed with EBV. Fibroblasts designated 149BR (ECACC catalogue no. 90011807) have been isolated from the same individual . This has been confirmed by STR profiling at ECACC. T cells from the same sample have been used for cellular radiosensitivity studies and cell survival analysis after UV-irradiation.

Also Known As:

Species: Human

Tissue: Blood

Growth Properties: Suspension

Morphology: Lymphoblastoid

Growth Medium: RPMI 1640 + 4mM Glutamine + 200 µg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).

Subculturing Procedure: Maintain cultures between 3-9 x 100,000 cells/ml, dilute every 1-2 days to 3 x 100,000 cells/ml; 5% CO2, 37°C. Cells form floating aggregates.

Release Conditions: No

Depositor: Dr. J. Cole, MRC Cell Mutation Unit, University of Sussex, Falmer, Brighton, East Sussex, BN1 9RR

Originator: Yes

References: Green MH, Arlett CF, Cole J, Harcourt SA, Priestley A, Waugh AP, Stephens G, Beare DM, Brown NA, Shun-Shin GA 1991 Comparative human cellular radiosensitivity: III. Gamma-radiation survival of cultured skin fibroblasts and resting T-lymphocytes from the peripheral blood of the same individual. Int J Radiat Biol. 59(3):749-65 PMID: 1672363.

Hyperlink to ECACC Cell Line Data Sheet: 98062317