LB7
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Description
Description: Established by EBV transformation of mononuclear cells from the peripheral blood of a 51 year old male. T cells from the sample have been used as normal control for DNA repair studies and mutation analysis.
Also Known As:
Species: Human
Tissue: Blood
Growth Properties: Suspension
Morphology: Lymphoblastoid
Growth Medium: RPMI 1640 + 4mM Glutamine + 200 µg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).
Subculturing Procedure: Maintain cultures between 3-9 x 100,000 cells/ml, dilute every 1-2 days to 3 x 100,000 cells/ml; 5%CO2; 37°C. Cells form floating aggregates.
Release Conditions: No
Depositor: Dr. J. Cole, MRC Cell Mutation Unit, University of Sussex, Falmer, Brighton, East Sussex, BN1 9RR
Originator: Yes
References: Mutation Res. 1994; 316: 91, Env Molec Mutagenesis 1997; 29:36, Env Molec Mutagenesis 1997; 30:97
Hyperlink to ECACC Cell Line Data Sheet: 98062312
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