PC-9 (formerly known as PC-14)
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Description
Description: Derived from a human adenocarcinoma from lung tissue which remains differentiated. PC-14 was originally deposited with the Riken BioResource Center as a cell line derived from human lung adenocarcinoma (undifferentiated type) in 1989. The RIKEN BioResource Center subsequently informed the European Collection of Cell Cultures (ECACC) that short tandem repeat (STR) DNA profile analysis, undertaken in collaboration Immuno-Biological Laboratories Co., Ltd Japan, has shown this cell line to be identical to PC-9 a cell line derived from human lung adenocarcinoma (differentiated type). The misidentification occurred prior to the cell line being deposited at the RIKEN BioResource Center. The name of the cell line has been changed to PC-9 to reflect this finding.
Also Known As:
Species: Human
Tissue: lung
Growth Properties: Adherent/suspension
Morphology: Heterogeneous mixture of round cells and spindle shaped cells
Growth Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Seed cells at 2-4 x 10, 000 cells/cm²; 5% CO2; 37°C. Remove attached cells by brief treatment with 0.25% trypsin or trypsin/EDTA.
Release Conditions: No
Depositor:
Originator: No
References: None specified by depositor
Hyperlink to ECACC Cell Line Data Sheet: 90071810
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