PORTHOS
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Description
Description: Derived from porcine thyroid. The cells have been adapted to grow in low serum (0.1%) media.
Also Known As:
Species: Porcine
Tissue: thyroid
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: RPMI (1:1) + 10µg/ml Insulin + 10mM Hydrocortisone + 5µg/ml Transferrin + 10ng/ml glycyl-L-histydyl-L-lysine acetate + 10ng/ml somatostatin + 10mU/ml Thyroid Stimulating Hormone (TSH) + 2mM Glutamine + 0.1% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 seeding at 2 x 10,000 cells/cm² using 0.05% trypsin/0.025% EDTA in Puck F saline; 2.5% CO2; 37°C.
Release Conditions: No
Depositor: Dr G Fayet/Dr S Hovsepian, Laboratoire de Biochimie Medicale, Faculte de Medecine, Marseille, F-13385
Originator: Yes
References: Fayet G and Hovsepian S (1985) In: Progress in Endocrine Research and Therapy, vol 2, Thyroglobin – The Prothyroid Hormone (eds) Burrow GN and Eggo M. Raven Press, NY. pp211-224.
Hyperlink to ECACC Cell Line Data Sheet: 93122323
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