RAT-2
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Description
Description: RAT-2 was derived from a subclone of a 5′-bromo-deoxyuridine (BUdR)-resistant strain of the Fischer rat 3T3-like cell line RAT-1. RAT-2 lacks appreciable levels of nuclear thymidine kinase, is highly transfectable by exogenous DNA and is phenotypically normal.
Also Known As:
Species: Rat
Tissue: embryo
Growth Properties: Adherent
Morphology: Fibroblast-like
Growth Medium: DMEM + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-4×10, 000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: Virology 1981;113:408-411
Hyperlink to ECACC Cell Line Data Sheet: 94050409
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