RAW 264.7
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Description
Description: Established from an ascites of a tumour induced in a male mouse by intraperitoneal injection of Abelson Leukaemia Virus (A-MuLV). Cells will pinocytose neutral red and phagocytose zymosan. Cells capable of antibody dependent lysis of sheep erythrocytes and tumour targets. Growth inhibited by LPS.
Also Known As:
Species: Mouse
Tissue: Blood
Growth Properties: Semi-adherent
Morphology: Macrophage
Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:8 i.e. seeding at 2-4×10,000 cells/cm²; 5% CO2; 37°C. Use cell scrapers to remove attached cells. Cells are semi-adherent, i.e. some cells grow in suspension, some loosely attach to the surface and others flattened out and attached to the flask. Cells should not be allowed to overgrow and become confluent as this can lead to loss of the flattened adherent cell characteristic.
Release Conditions: No
Depositor:
Originator:
References: J Immunol 1977;119:950; Cell 1978;15:261
Hyperlink to ECACC Cell Line Data Sheet: 91062702
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