Description: RCC BA85#21 (ELTHEM) is a renal tumour cell line which was established from a primary tumour in a 62 year-old male patient with a clear cell carcinoma. The tumour tissue of the primary lesion was composed principally of clear cells with alveolar/tubular arrangement. The immunogenicity of the RCC BA85#21 (ELTHEM) cell line has been experimentally proven by in vitro stimulation under autologous and allogeneic conditions using dentritic cells loaded with cell lysate, cytotoxic assay and by the Elispot technique. Characterisation of RCC BA85#21 (ELTHEM) by phenotypic and molecular assays such as cytochemistry, flow cytometry, microarray analysis Real-Time PCR microsatellite instability and loss of heterozygosity is described.

Also Known As: ELTHEM

Species: Human

Tissue: Kidney

Growth Properties:

Morphology: Polygonal

Growth Medium: RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Subculturing Procedure: Split sub-confluent cultures (90%) 1:6 with media changes every 2-3 days. Inoculate new cultures at 4×10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Release Conditions: No

Depositor: Professor Elena Ranieri, Universita’ degli Studi Di Foggia, Italy.

Originator: Yes

References: 2005 Title : “Renal carcinoma cell line and use thereof. Industrial Patent No. MI2005 A002018, 21.10.05. Inventors: E Ranieri, L Gesualdo, W Herr, M Battaglia. Applicants: University of Bari, University of Foggia.

Hyperlink to ECACC Cell Line Data Sheet: 13051601