Ri-1
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Description
Description: The cell line Ri-1 has been established from the peripheral blood of a 48-year-old female patient with B-cell lymphoma. This EBV-negative cell line with 14q+ abnormality was shown to be at an intermediate stage of B-cell differentiation. Ri-1 expresses surface and cytoplasmic immunoglobulin (IgM kappa)”, HLA-DR, CD20, CD38 and FMC7. Rearranged heavy-light chain genes and one rearranged kappa light-chain gene was detected as well as a kappa and lambda light-chain gene in germ-line configuration.
Also Known As:
Species: Human
Tissue: blood
Growth Properties: Suspension
Morphology: Lymphoblastoid
Growth Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Maintain cultures between 3-9×100,000 cells/ml; 5% CO2; 37°C. Cell growth is very slow after resuscitation; large amounts of debris present.
Release Conditions: No
Depositor: Dr J V Melo, Department of Haematology, RPMS, Hammersmith Hospital, London
Originator: Yes
References: Int J Cancer 1987;39:89
Hyperlink to ECACC Cell Line Data Sheet: 96090512
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