RK13
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Description
Description: RK13 has been derived from kidney cells of a 5 week old rabbit. The cell line has been shown to be susceptible to pseudorabies virus, rabbitpox virus, myxomatose virus, vaccinia and EAV. Sensitivity to other viruses such as Simian adenovirus and rubella has been reported as well with the production of cytopathic effect (CPE). RK13 has been successfully treated with thiamutin for eradication of mycoplasma. The Bovine Viral Diarrhoea Virus (BVDV) status of this cell line was previously described as negative. Results of recent multiple molecular tests for BVDV that were undertaken by two independent testing laboratories were equivocal. This means that we cannot confirm the BVDV status of this cell line.
Also Known As:
Species: Rabbit
Tissue: Kidney
Growth Properties: Adherent
Morphology: Epithelial-like
Growth Medium: MEM(HBSS)+ 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) seeding at 1-3 x10, 000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Monolayer is confluent after 48-56 hrs and stable for at least 21d; small syncytia in elder cultures are typical.
Release Conditions: No
Depositor:
Originator: No
References: Lancet 1963; 2:640
Hyperlink to ECACC Cell Line Data Sheet: 00021715
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