Description: SK-GT-2 was established from a primary adenocarcinoma of gastric fundus from a 72-year-old Hispanic male previously treated for squamous cell carcinoma with radiation therapy and radical neck dissection. Pathology of recurrence (1 year) demonstrated ulcerating poorly differentiated adenocarcinoma of the stomach invading spleen and epigastic fat (T4) with metastases to perisplenic and perigastric nodes (N1). SK-GT-2 was found to be tumorigenic in athymic nu/nu mice. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

Also Known As:

Species: Human

Tissue: Oesophagus

Growth Properties: Adherent

Morphology: Epithelial

Growth Medium: RPMI-1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Population doubling approx 44hrs.

Release Conditions: No

Depositor:

Originator:

References: Boonstra JJ, van Marion R, Beer DG, Lin L, Chaves P, Ribeiro C, Pereira AD, Roque L, Darnton SJ, Altorki NK, Schrump DS, Klimstra DS, Tang LH, Eshleman JR, Alvarez H, Shimada Y, van Dekken H, Tilanus HW, Dinjens WN. 2010 Verification and unmasking of widely used human esophageal adenocarcinoma cell lines. J Natl Cancer Inst. 102(4):271-4.PMID: 20075370.

Hyperlink to ECACC Cell Line Data Sheet:  11012008