SK-N-BE(2)
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Description
Description: Established from a bone marrow biopsy of a 22-month-old male with disseminated neuroblastoma in 1972. The morphology varies with sub-population from cells with long processes through small, flattened, epithelioid cells. Dense cultures tend to form loosely adherent aggregates. They are multipotential with regard to neuronal enzyme expression and display a high capacity to convert tyrosine to dopamine.
Also Known As:
Species: Human
Tissue: neural
Growth Properties: Adherent
Morphology: Epitheilioid & neuroblastoid
Growth Medium: EMEM (EBSS) + 1% Non Essential Amino Acids (NEAA) : Ham’s F12 (1:1) + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:10 to 1:25 i.e. seeding at 2-4×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor: Dr R A Ross and Dr BA Spengler, Department of Biological Sciences, Fordham University, NY
Originator: Yes
References: Cancer Res 1978;38:3751; Life Sciences 1991;50:7; Science 1976;191:185; J Natl Cancer Inst 1976;57:683
Hyperlink to ECACC Cell Line Data Sheet: 95011815
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