SK-PN-DW
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Description
Description: SK-PN-DW has been derived from a male patient with a primitive neuroectodermal tumour that expresses cholecystokinin (cck) RNA and synthesises substantial amounts of pro-cck. SK-PN-DW is able to process the pro-hormone, producing immunoreactive cck-like material plus a peptide that immunochemically and chromatographically resembles intact cck c-terminal extension peptide. Cells have a tendency to float.
Also Known As:
Species: Human
Tissue: neural
Growth Properties: Semi-adherent
Morphology: Polygonal
Growth Medium: DMEM or EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor: Mrs C Helson, St Agnes Hospital, 305 North St, White Plains, New York
Originator: Yes
References: J Clin Endocrin and Metab 1989;69(2):411; Cancer Genet Cytogenet 1987;24:75
Hyperlink to ECACC Cell Line Data Sheet: 94092301
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