SV3T3B
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Description
Description: A 3T3 cell line doubly transformed by polyoma then SV40 viruses, probably the fore-runner of SV-T2 (ECACC catalogue no. 90030801). Demonstrates a high degree of contact inhibition.
Also Known As:
Species: Mouse
Tissue: embryo
Growth Properties: Adherent
Morphology: Fibroblast
Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10, 000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: Virology 1965;27:179; J Cell Physiol 1968;12:141; Science 1968;162:1024
Hyperlink to ECACC Cell Line Data Sheet: 85011409
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