SW 480
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Description
Description: Derived from a grade 3-4 colon adenocarcinoma. The initial cultures contained a mixture of epithelial and bipolar cells, but subsequently epithelial cells predominated. The cells produce CEA.
Also Known As:
Species: Human
Tissue: colon
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:8 i.e. seeding at 2-3×10, 000 cells/cm² using 0.25% trypsin; 37°C. Medium change every 4-5 days. CO2 is not required.
Release Conditions: No
Depositor:
Originator: No
References: Cancer Res 1976;36:4562
Hyperlink to ECACC Cell Line Data Sheet: 87092801
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