T84
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Description
Description: Derived from a lung metastasis of colon carcinoma in a 72 year old male. Tumour tissue was inoculated sub-cutaneously and serially transplanted in BALB/c nude mice and subsequently established in in vitro culture. The histological characteristics of the tumour were maintained throughout the transplantation procedure. They have receptors for a range of peptide hormones and neurotransmitters. The cells grow as monolayers, exhibit tight junctions and desmosomes between adjacent cells.
Also Known As:
Species: Human
Tissue: Colon (lung metastasis)_
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: Ham’s F12 + DMEM (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 – 1:4 i.e. seeding at 1-3 x 10,000 cells / cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Cells grow slowly and may not form a complete monolayer, maintain at high density with a minimum of 25% confluency.
Release Conditions: No
Depositor:
Originator: No
References: Proc Natl Acad Sci, USA 1980;77:3464; Am J Physiol 1984;246:G204
Hyperlink to ECACC Cell Line Data Sheet: 88021101
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