TM4
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Description
Description: From primary culture of sertoli cell enriched preparations from normal testes of a 11-13 day old BALB/c nu/+ mice. Cells respond to FSH but not LH; Plasminogen activator secretion is stimulated by FSH and also retinoic acid. Note: only distributed frozen.
Also Known As:
Species: Mouse
Tissue: testis
Growth Properties: Adherent
Morphology:
Growth Medium: Ham’s F12 + DMEM (1:1) + 2mM Glutamine + 5% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:20 to 1:200 i.e. seeding at 1×10000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C. Cells resuscitated from frozen stock should be split 1:3 at the first passage and thereafter as above.
Release Conditions: No
Depositor:
Originator: No
References: Bio Repro 1980;23:243; Ann NY Acad Sci 1982;383:44; J Biol Chem 1984;259:3117
Hyperlink to ECACC Cell Line Data Sheet: 88111401
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