WILCL
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Description
Description: Normal B cell line of a patient with a lung tumour. LUDLU-23, a lung squamous cell carcinoma, is also derived from the same patient.
Also Known As:
Species: Human
Tissue: blood
Growth Properties: Aggregates in suspension
Morphology: Lymphoblast
Growth Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Maintain cultures between 3-9×100,000 cells/ml; 5% CO2; 37°C. Cells grow as large cell aggregates; resuscitate in 8ml media with flask standing up until cells start to proliferate well.
Release Conditions: No
Depositor: Dr P Rabbitts, MRC, Clinical Oncology and Radiotherapeutics Unit, Cambridge
Originator: Yes
References: None specified by depositor
Hyperlink to ECACC Cell Line Data Sheet: 89120565
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