WRC
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Description
Description: Derived from the parent line LLC-WRC 256, which was established in 1954. The cells are tumorigenic in suckling rats and support the growth of HSV, PRV and Vaccinia viruses.
Also Known As:
Species: Rat
Tissue: unknown
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor: Dr E I Levene, Department of Pathology, University of Cambridge
Originator: Yes
References: Anat Rec 1956;124:490
Hyperlink to ECACC Cell Line Data Sheet: 88031148
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