Description: Established from a primary tumour of the right eye of a 2.5 year old female Caucasian. The cells grow in clusters and exhibit reverse transcriptase activity.

Also Known As:

Species: Human

Tissue: Retina

Growth Properties: Suspension

Morphology:

Growth Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: The percentage of viable cells is generally low upon resuscitation from frozen ampoules (often less than 50%). However the cells should recover in a few days. When starting from a frozen ampoule the cryoprotectant should be removed. Add thawed cells to a conical based centrifuge tube e.g. 15ml tube; slowly add 4 ml of culture medium to the tube. Take a sample of the cell suspension, e.g. 100ul, to count cells. Centrifuge the cell suspension at low speed i.e. 100 – 150 x g for a maximum of 5 minutes. Remove medium and resuspend the cell pellet at a density of 5 x 100,000 cells/ml in fresh medium. Incubate flask at 37°C; 5-7% CO2. Check daily. Maintain cultures between 4-9×100,000 cells/ml; 5% CO2; 37°C.When in culture, stand flask up until cells show growth in large clumps. After approximately 3 days the flask can be laid down. Requires 5% DMSO and 95% foetal bovine serum (FBS) as cryoprotectant.

Release Conditions: No

Depositor:

Originator: No

References: J Nat Cancer Res 1974;53:347

Hyperlink to ECACC Cell Line Data Sheet: 86093003