Eos-HL-60
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Description
Description: Eos-HL-60 is a variant of the promyelocytic cell line HL60 (ECACC catalogue no. 85011431) established by sub-cloning in methylcellulose. Although the cells are capable of reverting to the parental phenotype, most passages maintain a high degree of eosinophil differentiation.Eos-HL-60 and HL-60 have been shown to be identical genetically by STR profiling
Also Known As:
Species: Human
Tissue: blood
Growth Properties: Suspension
Morphology: Lymphoblastoid
Growth Medium: IDMEM + 2mM Glutamine + 0.5mM Butyric acid + 10% Foetal Bovine Serum (FBS); pH7.6; add Butyric acid to the medium just before use.
Subculturing Procedure: Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO2; 37°C.
Release Conditions: No
Depositor: Dr D W Golde, Memorial Sloan-Kettering Cancer Centre, New York
Originator: Yes
References: Blood 1986;67:1433; Blood 1992;80:788
Hyperlink to ECACC Cell Line Data Sheet: 96100920
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