HT29/219
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Description
Description: Subclone of parent HT-29 which was established from an adenocarcinoma from a 44 year old Caucasian female. Tumorigenic in nude mice and has ability to grow in agar.
Also Known As:
Species: Human
Tissue: Colon
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: Proc Soc Exp Biol Med 1981;166:107; J Nat Cancer Inst 1977;58:1743; Fogh J. Trempe G: New Human Cell Line. In Human Tumour Cells in vitro. New York: Plenum Press 1975;p115
Hyperlink to ECACC Cell Line Data Sheet: 85061109
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