LB687

CODE: 98062335
Human lymphoblastoid", Xeroderma pigmentosum complementation," EBV-transformed

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Description

Description: Established by EBV transformation of mononuclear cells from a peripheral blood sample from a 30 year old male patient. The patient was diagnosed with Xeroderma pigmentosum complementation group A, a rare, autosomal recessive disorder with DNA-repair deficiency.

Also Known As:

Species: Human

Tissue: Blood

Growth Properties: Suspension

Morphology: Lymphoblastoid

Growth Medium: RPMI 1640 + 4mM Glutamine + 200 µg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).

Subculturing Procedure: Maintain cultures between 3-9 x 100,000 cells/ml, dilute every 1-2 days to 3 x 100,000 cells/mls; 5% CO2; 37°C. Cells form floating aggregates.

Release Conditions: No

Depositor: Dr. J. Cole, MRC Cell Mutation Unit, University of Sussex, Falmer, Brighton, East Sussex, BN1 9RR

Originator: Yes

References: None specified by depositor

Hyperlink to ECACC Cell Line Data Sheet: 98062335

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