ST
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Description
Description: The cell line was established in 1960 from the trypsinised testis of a normal 80-90 day old porcine foetus. The cells support the growth of various porcine parvo- and entero-viruses, and also pseudorabies virus. Also useful in the propagation and isolation of transmissible gastro-enteritis of swine.
Also Known As:
Species: Porcine
Tissue: testis
Growth Properties: Adherent
Morphology:
Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10, 000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: Can J Comp Med Vet Sci 1966;30:190
Hyperlink to ECACC Cell Line Data Sheet: 92040221
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